Discovery of anti-tuberculosis and antibiotic compounds from microorganisms in the sea of Northern Central region (from Northern Central to Mid-Central Vietnam) Project’s code: VAST.TĐ.DLB.04/16-18 Belonging to Focal Point

Project leader's name
Assoc. Prof. Dr. Habil Pham Van Cuong
Research hosting institution
Institute of Marine Biochemistry
Project duration
2016 - 2018
́Project’s budget
5800 million VND
Classify
Excellent
Goal and objectives of the project

Investigation of anti-tuberculosis and antimicrobial constituents from microorganisms in the Middleof Vietnam’s Sea.

Main results

- Collection of154 marine samples at three areas (Thanh Hoa - Quang Binh - Quang Tri; Hue- Quang Nam - Da NangandQuang Ngai - Binh Dinh - Phu Yen) of the Middleof Vietnam’s Sea.
- 121 bacteria strains were isolated from 154 marine samples, including 34 strains of Thanh Hoa - Quang Binh - Quang Tri, 46 strains of Hue- Quang Nam - Da Nangand 41 strains of Quang Ngai - Binh Dinh - Phu Yen.
- All isolated strains were fermented at small scale which were screened for their antimicrobial and antituberculosis activity. Accordingly, 15 strongest active strains were selected for their taxonomic identification, fermentation in large scale and secondary metabolites analysis.
- All extracts of culture broths of the 15 bacteria strains were fractionated by chromatography method that led to the isolation and characterization of 97 compounds, including 4 new secondary metabolites and 4 new compounds from a natural source. Structures of new compounds were indicated below.

- All the isolates were evaluated for their antimicrobial and antituberculosis activity. The results showed that 43 compounds had antimicrobial activity, compound G246-1exhibited remarkable antibacterial activityagainst 7 microbial strains, 5compound(G212-6, G212-7, G246-2, G263-1, G263-12)had antimicrobial activity against 6/7 microbial strains, 4compound(G212-4, G212-8, G278-11, G278-17) had antimicrobial activity against 5/7 microbial strains, 4compound(G212-3, G212-5, G263-4, G361-11)had antimicrobial activity against 4/7 microbial strains. For the antituberculosis activity, 3 compounds exhibited an inhibition activity againstH37Rv.\

Some images of the project:

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Novelty and actuality and scientific meaningfulness of the results

- First discovery of new secondary metabolites from marine bacteria of the Middleof Vietnam’s Sea. Accordingly, 4 new secondary metabolites (G212-2,G212-3,G246-1 and G246-2)and 4 new compounds from a natural source (G212-1,G278-16,G278-17and G261-4)were isolated and characterized.
- The actinomycete strain Streptomyces sp G212was determined to be able to produce Germicidine A (G212-4), Germicidine B (G212-5), which is currently used as an antibiotic drug. This discovery could led to the optimization of fermentation condition for the production of Germicidine A andGermicidine B by the strain Streptomyces sp G212.
- 43 compounds had antimicrobial activity and 3 compounds exhibited an inhibition activity against Mycobacterium tuberculosisH37Rv.
- The results of this project encourage a research of bioactive compounds from marine microorganisms for their pharmacological use.

Products of the project

- Training: one Master and two PhD student
- 9 articles in the domestic scientific journals and 03 articles in international scientific journals (ISI).
1. Duc Tuan Cao, Van Hieu Tran, Van Nam Vu, Huong Doan Thi Mai, Thi Hong Minh Le, Thi Quyen Vu, Hung Huy Nguyen, Van Minh Chau & Van Cuong Pham. Antimicrobial metabolites from a marine-derivedActinomycete Streptomyces sp. G278. Natural Product Research, 2018, DOI 10.1080/14786419.2018.1468331 (online)
2. Duc Danh Cao, Thi Quynh Do, Huong Doan Thi Mai, Quyen Vu Thi, Hong Minh Le Thi, Dang Thach Tran, Van Minh Chau, Cong Thung Do and Van Cuong Pham. Antimicrobial Lavandulylated Flavonoids from a Sponge-Derived Actinomycete. Natural Product Research, 2018 (accepted)
3. Duc Tuan Cao, Thuy Linh Nguyen, Van Hieu Tran, Huong Doan Thi Mai, Quyen Vu Thi, Mai Anh Nguyen, Hong Minh Le Thi, Van Minh Chau and Van Cuong Pham, Synthesis, Structure and Antimicrobial Activity of Novel Metabolites from a Marine Actinomycete in Vietnam’s East Sea, Natural Product Communication, 2018 (accepted)
4. Cao Duc Tuan, Tran Van Hieu, Doan Thi Mai Huong, Vu Van Loi, Le Thi Hong Minh,Vu Thi Quyen, Brian T. Murphy, Nguyen Quoc Vuong, Pham Van Cuong. Compounds from culture broth of marine actinomycete Dietzia sp. G113. Vietnam Journal of Chemistry, 2017, 55 (6e), 17-22.
5. Cao Duc Tuan, Vu Van Nam, Tran Van Hieu, Doan Thi Mai Huong, Vu Thi Quyen,Nguyen Mai Anh, Le Thi Hong Minh, Brian T. Murphy, Nguyen Quoc Vuong,Pham Van Cuong. Secondary metabolites from marine actinomycete Streptomyces sp. G212. Vietnam Journal of Chemistry, 2017, 55 (6e), 85-89.
6. Cao Duc Danh, Vu Van Nam, Doan Thi Mai Huong, Tran Dang Thach, Vu Thi Quyen,Nguyen Mai Anh, Le Thi Hong Minh, Brian T. Murphy, Nguyen Quoc Vuong,Pham Van Cuong. Secondary metabolites from marine actinomycete Streptomyces sp. G266. Journal of Chemistry, 2017, 55 (6e), 107-110.
7. Cao Duc Danh, Truong Bich Ngan, Phan Thanh Nam, Doan Thi Mai Huong,Tran Dang Thach, Le Thi Hong Minh, Nguyen Mai Anh, Brian T. Murphy,Nguyen Quoc Vuong, Pham Van Cuong. Secondary metabolites from Micromonospora sp. G233. Vietnam Journal of Chemistry, 2017, 55 (6e), 135-139.
8. Cao Duc Tuan, Tran Van Hieu, Doan Thi Mai Huong, Le Thi Hong Minh, Vu Thi Quyen,Nguyen Mai Anh, Brian T. Murphy, Nguyen Quoc Vuong, Pham Van Cuong. Secondary metabolites produced by marine actinomycette Streptomyces sp. G278. Vietnam Journal of Chemistry, 2017, 55 (6e), 145-149.
9. Cao Duc Danh, Truong Bich Ngan, Doan Thi Mai Huong, Tran Dang Thach,
Le Thi Hong Minh, Nguyen Mai Anh, Chau Van Minh, Pham Van Cuong. Secondary metabolites from Micromonospora sp.G244. Vietnam Journal of Chemistry, 2018, 56 (4e), 203-207.
10. Cao Duc Tuan, Le Thi Hong Minh, Vu Thi Quyen, Nguyen Mai Anh, Doan Thi Mai Huong, Chau Van Minh, Pham Van Cuong. Identification and antimicrobial activity of actinomycetes strains isolated from samples collected in the coastal area of Hue, Da Nang and Quang Nam provinces, Vietnam. Vietnam Journal of Biotechnology 15(4): 737-744, 2017
11. Cao Duc Danh, Cao Đuc Tuan, Vu Thi Quyen, Nguyen Mai Anh,
Doan Thi Mai Huong, Pham Van Cuong, Chau Van Minh, Le Thi Hong Minh. Identification and antimicrobial activity ofactinomycetes strains isolated from marinesamples in the coastal area of Thanh Hoa - QuangBinh – Quang Tri. Vietnam Journal of Science and Technology56(4), 1-10, 2018. DOI: 10.15625/2525-2518/56/4/10663
12. Cao Duc Danh, Phi Thi Dao, Doan Thi Mai Huong, Tran Dang Thach, Nguyen Mai Anh, Le Thi Hong Minh, Tran Tuan Anh, Pham Van Cuong, Cyclopeptides from marine actinomycete Streptomyces sp. G261, Vietnam Journal of Chemistry, 2018, 56(5), 570-573.
- 1 patent are accepted as correct forms byNational Office of Intellectual Property of Viet NamMinistry of Science and Technology.
- Technological products:
1. Pure compounds isolated and their spectra were stored at Department of Organic Synthesis – Institute of Marine Biochemistry.
2. 121 bacteria strains were stored at Institute of Marine Biochemistry and registered at Center for Culture Collection and Genetic Resourse Conservation of Microorganisms - Institute of Biotechnology - VAST.

Recommendations

Completion of this project

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